Incidents in the Life of a Slave Girl, by Harriet A. Jacobs, Writing Essay

Incidents in the Life of a Slave Girl, by Harriet A. Jacobs, Writing as Linda Brent - Essay Example When that mistress, Margaret Horniblow died, everything changed for the worse for Harriet, who was willed to Dr. Norcom, the Dr. Flint in 'Incidents'. While he did not actually whip her, he sexually harassed the girl, an issue she seeks to expose in order to enlist the understanding and action of Northern white women; that a slave woman has no freedom either as a person or a woman. Flint makes this clear, often. It is difficult to select only three critical incidents in her life, but one has to be the suffering endured at the hands of both Dr. Flint and his jealous wife. The effect of this caused her to abandon her moral stance regarding purity, and almost lost her the allegiance of her grandmother. By the taking of a white lover, Lawyer Sands, (Samuel Treadwell Sawyer) and bearing him two children, she may be considered to be enslaving herself further. She explains that such action was a means of self protection, for Flint still tormented her, wishing to set her up as his mistress in a cottage somewhere, and she could not succumb to this form of enslavement. Instead, she chose to use her sexuality as a means of escape. The children do however, belong to Flint, but with the hope of them being set free, she removes herself, going into hiding for seven years in her grandmother's attic. This is another important, central issue to the story, for she shows her audience that she cannot be the mother to them she desires to be, but will sacrifice this for their freedom. The happy outcome of these actions means that Sands is able to buy the children from Flint, with the help of some subterfuge. "The darkest cloud that hung over my life had rolled away. Whatever slavery might do to me, it could not shackle my children, If I fell a sacrifice, my little ones were saved." (Chap. 19, p. 166) In Chapter 29, when she is ready to escape, after delays and prevarication, there is a bitter-sweet quality in her discussion with her son, who says, "Mother, I'm glad you're going away. I wish I could go with you...I have been so afraid they would come and catch you" (Chap. 29 p. 234) The escape, and what comes after it, signify a third important incident for Harriet/Linda, bringing about, as it does, a faith in the kindness of others (the Bruce family) but a bitter understanding that the Fugitive Law 1851 (Chap. 40) still robs her and her people of freedom. Regarding the standards of womanhood in 19th century America, Jacobs is aware of these and how she is deprived of the freedom to adhere to them. The ideal image of womanhood was that of motherhood, traditional homemaker, one who creates a place of love and security for her family, and who possesses the virtues of purity, piety and submission. At the same time, while nurturing the family, she must use her moral values, judged higher than the male of the species, to teach and develop her children. Jacobs is driven by this maternal instinct, the desire to secure freedom for her children, and to nurture them in a secure family unit, spiritually and physically. These elements are what she portrays to show evidence of 'respectability' in that society. On the other hand, the contradiction lies

History of Nail Care Essay Example | Topics and Well Written Essays - 750 words

History of Nail Care - Essay Example In ancient Egypt, the color of the nails of an individual determined his/her status and class – it was an emblem of importance. While individuals of lower rank were only permitted to wear pale colors on their nails; queens and kings wore deep red (Trapp, 13 and David, para1). During the reign of Queen Nefertiti, red nail colors symbolized the status of royals. Furthermore, the famous queen Cleopatra who ruled Egypt always painted her nails with a rusty red color, which was only set aside for the queen (HubPages Inc., para2). Similarly, in ancient China, fingernails played an important role in identifying the nobles. Individuals who held high status wore long nails as a symbol of their status, and their nail color revealed their social class. Silver and gold colors were an emblem of highest power and rank, while red and black represented boldness and strength. The common people, on the other hand, used pale tones, and they symbolized weakness. The Chinese came up with a nail polish made from gum Arabic, beeswax, gelatin, and egg whites around 3,000 B.C. In Chou Dynasty, Chinese individuals of highest status and ranks painted their nails with silver and gold polish in 600 B.C. During the 15th Century, Chinese Ming Dynasty’s leaders painted their nails red and black. Before the battle in Babylon, early Rome and Egypt, military commanders spent hours curling and lacquering their hair and painting their nails and their lips with the same color (HubPages Inc., para3 and Trapp, 13). Nail care started being a part of glamour and fashion in the 19th century. With the technological and social advancements, new nail care tools started surfacing in Europe. A foot doctor by the name Sitts devised a wooden manicure apparatus in the year 1830. Prior to his invention, people used acid, scissors, metal rod, among others for manicure. During this period, slightly pointed nail styles or almond-shaped nails were common. The

Data Locations and Security Issues in Cloud Computing

Data Locations and Security Issues in Cloud Computing ANURAG JAIN   JITENDRA TAWARI   SANDEEP SONI ABSTRACT-: Cloud computing is one of todays most important technology because in this technology cost low, exciting and flexible. It is the most important technology of growing industrial areas. It allows user to access applications, documents etc., that consist location of our own computer or other Internet-connected devices. Data is stored remotely from a location.it can be stored in any location. Within the fast growing of cloud computing technology, Data security becomes more and more important. Cloud Computing offers many benefits to the enterprise there are also many issues as with any new technology. One of the main issues relates to the secure and positive result of customer data in terms of its location, relocation, availability and security .The aim is to provide some useful information and security issues for organizations preparing to migrate to the Cloud to take advantage of this computing technology. In this research paper we have proposedan encryption algorithm to solve data loca tion security issues. Keywords-:cloud computing, security of data ,data location, internet, data privacy, encryption algorithm. Introduction cloud computingrises upthe fieldinanIT. Itsattributedto operation forward as employment up to the information to the cloud based hardware and system .In a cloud computing data are transferred betweenthe clientand the server .now a day cloud computing recently technology because can store and retrieve the data on the cloud by using remote technology .Its providing the software platform and framework as a utility. Today all the it companystoreshis data on the cloud .thisfacility given by the cloud provider or cloud owner .so here is require to defend that data against illegal access and changing .security issue of datacontainsthreepoints.Thesepointsare integrity ,availability and secrecy. secrecy of the data by the using ofcryptography. in a moderndaycryptography is very famous technique. Data cryptography used to hide of the data in the form of image ,sound, text, video .during the transmission hackers cant access the our data because he will get only unreadable and meaningless. the cloud assures low price saving and given the high speed to user. the cloud technology a company quickly expand application where growth and contraction of the basic technology part can be obtained with the high and low of the trade .it can be gotten with the help of cloud supporter. such as Virtualization and grid computing, that permit application dynamically expand on the proper infrastructure. security service of the cloud -there are some type of service of the cloud to solve the security issue .these service are storage as a service (saas),software as a service(saas),platform as a service (paas),infrastructure as a service(iaas). (A)storage as A service- the cloud computingallowsa huge space that is probably infinite, and it’s ascending day by day .storage as a service authorizea cloud applicationto measurer besides bound service. Cloud computing technologyis requiredto obtain various needsfor hold the user data and information , add high capacity , stability operation. In this service the user areresponsibleto own data and hold their storage as a cloud trader proposal them the option of storing their data in the cloud .the user can access the data whenever hewants.due to the hostile character of  ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­Ã‚ ­the need of the cloud , no one method implement all in all. (B)software as a service (saas):-software as service is very famous technology. this technology also referred as software offered on requirement . its depends on multi-tenant design. software like that customer relation organization .the benefit of SaaS technique that the apps are locally run on your computer . user access can any application by using the cloud technology that user can never own from any browser .the saas technique support his user to increasing application day by day .with the help of saas technology user create and upload own data on the cloud .globally uploading data any one can access . c. platform as a service:- we are developed the software by tools and libraries given by the cloud. the user manage the software operation and design location. services offered through (PaaS) help the operation of applications exclusive of the cost and complication of buying and organization the basic hardware and software. PaaS services also contain enhancing the application life cycle processes similar to application design, application development, testing and deployment. It provides an communicate with high level of combination. That is largely in arrange to execute and analysis cloud applications. The user does not manage the overall communications but he control deployed applications and their configurations. Infrastructure as a Service(iaas) IaaS is a general which an organization outsources the equipment used to maintenance operations such as storage, hardware device, servers and networking components. The service provider keeps the equipment and is answerable for housing, running and maintaining . The customer typically pays on a . Features and modules of IaaS include: Efficacy computing service and billing model. Computerization of organizational tasks. Forceful scaling. Desktop virtualization. Policy-based services. Internet connectivity. Security issues:- Companies are fast moving onto cloud computing because they can currently usage the greatest resources present on the market in the flash of an eye and also decrease their actions’ cost radically. But as more and more information is moved to the cloud computing the security concerns have on-going to develop. (1). Data breaking is the major security issue. A trained hacker Cando simply and get into a client cross application and get into the client’s private data. (2). the Wasteful and damaged APIs and boundaries convert easy targets. IT companies that offer cloud services allow third party companies to modify the APIs and familiarize their personal functionality which in drive permits these companies to understand the internal workings of the cloud. (3). the Denial of Service (DoS) is also a main danger wherein the user is fixed partial or no access to his/her data. Companies now use cloud 24/7 and DoS can source huge increase in cost both for the user and facility provider. (4).the Connection eavesdropping means that a hacker can scan your online activities and replicate/replay a particular communication to catch into your private data. It can also central to the user to prohibited or uninvited sites (5).Data loss also is a new issue. A malicious hacker can wash out the data or some usual/man-made adversity can wipe out your data. In such cases having an offline copy is a big benefit. negligence of the service provider also able to direct data loss. (6). Compatibility between different cloud services is as well an issue. If a client decide to shift from one .cloud to one more the compatibility ensures that here is no loss of data. (7). Cloud also can be used in wrong purposes i.e. cloud exploitation. Due to the accessibility of most recent technologies on the cloud it can be used for high end calculations which cannot be complete on a normal computer. (8). Insufficient considerate of cloud technologies can guide to unidentified levels of risk. Companies transfer to cloud because it provides significant reduction in cost but if transfer is complete without proper background knowledge, the problems that happen can be still greater. (9). Insider theft in the appearance of a present or previous employee, a service provider, etc who is capable to use the data for harmful purposes. (10). Safe storage of encryption keys as well a problem. still if you are using encryption for improved security, safe maintenance of the key becomes an issue. Who should be the holder of the key? client seems to be alive the response but how diligent and careful can he/she be will choose the security of the data. The other aspects about the cloud security categorize of different type of threats about the cloud security are following Access The plan of cloud technology to offer information to the customer from several position whenever user wants. because a web technology cloud service enables the customer to access his data from wherever he want .its is valid to all the services being given by it. all the user should know that where data is stored. at the any condition client can request to server to delete his data after that the data should be deleted. command the cloud technology controlling of data is very necessary because if we will not control our data then the other user can access our data. so the controlling of data is able to be seen to a few member of the cloud service provider .this data is defies the stage of control. fulfilment: the authorities of the we have to need a law of govern to protection of data on the cloud because cloud service can cross several jurisdictions approximately the all world. if any user store his data in other country and its contain sensitive data .this data only whose person which have a authorities to access the data and some set of laws apply on the data . Data reliability: Data reliability in easy terms means that the data is protected and no one change the data without the uses agreement .this is a basic need of cloud computing .the reliability of data in a cloud is a very impertinent in a present there are many algorithm design to protection of data. inspection: with the help of inspection we can check the activities occurrence on the cloud. inspection is a mechanism that keep a log and list actions to help stop infringement. Privacy Breaches: The cloud service supplier should report to its client about any violate in security. The client has to the correct to know what is incident in his/her space. How does the service provider take care of this. secrecy: This ensures that the client data is kept secret. privacy is one feature of cloud storage security that will lift question in a ordinary users mind. Cloud as such is a public system and is susceptible to new threats, therefore, privacy is very important. planned work in this paper we have planned a structure .with the help of this planned structure we involve a file which is involved securing of data files through file encryption technique. the file whose presentation on the computer will be encrypted technique based on AES algorithm. if user wants to read this file then before the user download the uploaded encrypted after that he can read it. there are many benefit of AES algorithm . in this algorithm we are using an encryption key used by AES algorithm .the order of this algorithm is 128,192 and 256 bits AES algorithm is also a lot quicker than the RSA. Thus, its superior option for safety of data on the cloud . in an AES algorithm. the proposed system works only when an internet connection accessible. (1) file upload there are some steps for the file upload process are clarified in the block. (a) take on the user name or password from user If the user is genuine then connect with the cloud. else show the Certification error. (b) the first user asks to pick file to be uploaded on a cloud . (C) ask the password from user for the encryption . (D) sure the password and make a key from this password. (E interpolate the encryption algorithm . (F) Upload an file on to the cloud. (G) ask the user if he wants to delete the file which is uploaded. delete the user selected file (F) after that connection disconnect with the cloud. stage-1 we have to first check that the user is authentic or not . and after that system will accept the user name or password. if the user name or password is valid the system will be accepted else the username or password and not match the system show error message. stage-2 in a second stage we will check the cloud is stable because the file uploading in this section. The user will upload the file. which is he wanting. stage-3 in this stage the user ask the password for encryption process The user is optional to use long passphrases as their passwords. This password is used for creation of a key. stage-4 this stage is a very important for the system. in this stage generated a key for the encryption technique. we are using AES is a symmetric key algorithm .this key used for encryption or description technology. in this technology generated a key for password to a generated function. We suggest the use of Password Based Key Generation Function. Password Based Key generation Function uses iterations for a password to default checking. this process is called a key exertion. in this stage the system saves the password which is entered once and create a key for encryption. stage-5 in this stage we are applying Advanced Encryption Standard algorithm . the Advance Encryption Standard algorithm is useful to the simple text to make the secret message text. as a result the user can be rest secure the data protected from the a variety of threats the uploaded is data is encrypted which can be decrypted when the user enters the password which he entered for the period of the encryption process. stage-6 when the secret message text is created then upload the encrypted file on the cloud and concerned with the removal of the real simple text file from the memory. The option is given by the user to delete the unique file which is uploaded on the cloud. if the user dont want to delete this file the he has a second option retaining the unique file. We suggest that the unique file should be removed. This ensures that no illegal right to use will be built up to the simple text file stored in the system. The method of file upload can be shown in bellow: successful uploaded delete original file upload encryption file user encrypted file (2) download file there are some stage of file download process describe in this part. Stage 1 The identity of the user is validation process have in this stage and the collection of files that the user has uploaded on the cloud . The user is ask to choose any one of the files from the record. stage2- the user is asked for the password during the encryption of the file justification . entered password is complete in another stage. The secret message text file uploaded when the decrypted process will completed by the user and downloaded. the same password entered during the encryption. this is only motive that the password save for the duration of encryption process so the store password is valid. AES algorithm is a symmetric key algorithm so, it wants the same key to encrypt and decrypt the data. This procedure possible only if the same password is entered into the key creator job to make the key. stage 3- the means of the generated key, the AES algorithm to decrypt the uploaded secret message text. stage 4 the user Save this decrypted simple text in the user system memory. stage 5 the user is asked if he needs to remove the secret message text file uploaded on the cloud. If the user chooses to perform, remove the encrypted file from the file. the user does not desire to download any additional files from the cloud, log out of the user account and after that cut off the recognized link with the cloud. This is the very last stage of the download process. (A) we first Accept user name and password from the user If user is genuine set up connection through the cloud Else, show verification error (B). Ask user to pick file to be downloaded (C) the user ask to enter a password used for the decryption procedure (d) verify the legality of this password If the entered password is valid, the key is generated. Else. show the error message and refuse password (D) relate the decryption algorithm (E) Download the file commencing the cloud (F) the user ask if he wish for to delete the uploaded encrypted data file . Delete the file from the cloud which is encrypted. if user selects the delete choice (G) and cut off connection through the cloud . CONCLUSION Cloud computing is an puzzle anyone can get lost in. But separate like any new technology. the cloud computing is also a double enclosed stop. On one end lies the ability of fast technology, a huge array of applications to use, superficially infinite storage ability. On the other end story various security threats which develop with collective spaces such as break of privacy, impeding of data reliability and non-availability of data. In this paper, we have projected a structure which encrypts a file before it is uploaded on to the cloud. AES (Advanced Encryption Standard) is one of the most secure encryption measures and not many attacks are successful on data which is encrypted using AES. This application solves the problem of most, if not all. the threats that data kept in the cloud faces. We have framework also suggest the use of login id and password to ensure reliable and official access to a user’s data. Thus, if we used securely, cloud computing offers a user with wonderful profits and overcomes its only disadvantage of security threat.

How does H.G Wells build suspense in The Red Room? Essay -- English Li

How does H.G Wells build suspense in the red room? H.G. Wells "red room "is a pre-twentieth century suspense short story, which is a type of a Gothic story. I shall examine the various techniques that the writer has used to create and sustain suspense. For the first part of the essay I shall explain what a Gothic story is. A Gothic story is a type of romantic fiction that predominated in English literature in the last third of the 18th century, the setting to for which was usually a ruined Gothic, Castle or Abbey. The Gothic novel or Gothic romance, emphasised mystery and horror and was filled with ghost haunted rooms, underground passages and secret stairways. For this reason it is told the "red room" is a Gothic story, this could also explain how suspense in the "red room" was formed. Also if I look at the genre of the story, suspense is built at the beginning by H.G. Well's description of the man with the withered arm, the woman looking into the fire, the second old man and the description of the house: "Flags in the passage outside." "Door creaked on hinges." Also suspense is built by the description of the room, where the young man has to spend the night: "Steps up to it, were in a shadowy corner" This quote explains what the passage up to the "red room" was like. The mention of shadows in the description of the surroundings gives a feeling of evil, unfriendliness and dark unapproachable places. This also makes you feel on edge. In the structure of the story we constantly are made to be fearful of what we will find by the suggestive comment of the woman and the man with the withered arm. Some examples of the way the old woman and the man with the withered arm, second old man and ... ...nd brings tension back into the story. As the reader I know that the narrator is getting quite nervous: "A queer high note gets into his voice" These words were used to describe the darkness as candles were going out was so accurate. For example: "Like a ragged storm cloud sweeping out the stairs" Words used again to describe sudden darkness. The darkness became a: "Stifling embrace" too much for him to cope with. In my opinion H. G. Wells writes a very descriptive story. He manages throughout to convey a feeling of nervous tension and when he thinks that things are beginning to relax he introduces another unsettling point to the story. He keeps you on the edge of your seat and makes you wonder how the story will turn out. His use of descriptive words: "Deafens, darkens and overwhelms" makes the reader see this story in their minds my.

Fighting Bacterial Growth

Fighting Bacterial Growth The purpose of this lab was to determine the effectiveness of antiseptics, disinfectants, and antibiotics on bacteria. The hypothesis was that if bleach was used, it would be the most effective because bleach is commonly used to clean and disinfect various things. The variables that were tested were antibacterial soap and Scope mouthwash for the antiseptics; bleach and ammonia for the disinfectant; and Cipro, erythromycin, and tetracycline for the antibiotics.All of these chemicals were used on the bacteria M. luteus. Two Petri dishes were covered in the bacteria and split into four quadrants, in which each had a disc containing one of the chemicals stated above. One quadrant was left alone with no chemicals for the control group. The dishes were then left for the bacteria to grow, and once obtained again it was obvious that some of the bacteria was killed by the chemicals.In individual data, there was a zone of inhibition of 3mm in the antibacterial soap; 1 0mm in the Scope mouthwash; 2mm in the bleach; no zone of inhibition around the ammonia; 10mm in both the erythromycin and the tetracycline, and 15mm for the Cipro. The average length of the halo of inhibition in antiseptics was 8 mm in the E. coli, and 6 mm in the M. luteus. The average length of the halo of inhibition in the disinfectants was 12mm and 11mm respectively. For the antibiotics, it was 7mm and 9mm respectively.The data represented the hypothesis because for both the E. coli and the M. luteus, the largest zone of inhibition was in the disinfectant; and more specifically, the averages were 28mm in the E. coli and 18mm in the M. luteus in the bleach. In some Petri dishes, the bleach also killed bacteria in the other quadrants, indicating that it killed a lot of bacteria. This also affected some measurements for the other chemicals, because the zone of inhibition for the other chemicals around the bleach could have been caused by the bleach instead of the other chemical.An other error was that since these Petri dishes were left out for 2 days, there was re-growth in the bacteria in and around the zones of inhibition, like the ammonia in the individual data. In the lab, the chemicals were tested on bacteria to see how much of the bacteria will get killed. What kind of items then would create the most bacterial growth? If bleach was used on different kinds of bacteria, which kinds of bacteria would be most affected by the bleach, and which bacteria will be the least affected by the bleach?

Letter vs. Txtn

October 8, 2009 LW 102 9-11 am The Decease of Letter Writing Letter writing has all but disappeared in society today due to technologies such as e-mail and text messaging. Both of these technologies make the process of writing a letter and sending it through the mail much easier. Text messaging refers to messages being sent from one mobile phone to another. E-mail describes a system for sending and receiving messages over a computer network. Texting and E-mail have become part of people’s daily lives. Many people couldn’t imagine living without both. Even though texting and E-mailing are great things they are also making Americans lazy. When writing a letter you have to take the time to sit down and get your thoughts to process on paper. When writing letters you have to worry about correct spelling and vocabulary. Another big part of writing letters is grammar; it takes years sometimes to learn how to properly use the correct grammar and punctuation. Sometimes a major challenge in writing is being able to express an argument or a complete thought on paper. All of these things on top of technology contribute to why people don’t take the time to write letters anymore. E-mail also contributes to letter writing disappearing because even before texting became so popular there was e-mail. E-mail allows not only messages, but documents in little time. E-mail is convenient for PC users who are on the computer 24/7. E-mail allows you to send things like pictures and videos to another computer in less time it takes to mail a letter. E-mail is also cheap because most websites offer free e-mail addresses. Also most people who work in offices or from home rely on their e-mails very much to receive important information from their jobs quickly. Text messaging is another reason why letter writing has disappeared. Some people consider text messaging as their voice or identity. Texting is also one of the least expensive forms of communication and sends messages in less time than it takes to write. People text because it’s convenient and is like an extension of a PC. Unlike writing a letter, in text messaging you don’t have to worry about spelling because you can use short words and not have the correct spelling. Also in texting there is practically no grammar or punctuation. E-mail and text messaging are just two reasons why letter writing has deceased. These two technological advancements are much more convenient and easier to access. People are still communicating and sharing their original and personal thoughts with other people. So even though writing letters has disappeared somewhat people still express themselves in other ways.

Free Essays on In Womens Hand

Summary of â€Å"In Women’s Hands† During the 20th century, not only were many countries of Latin America undergoing stages of political instability with many governments having oppressive military rule, but women specifically were demanding more rights and respect inside and outside of the home. In Chile, women were becoming more assertive throughout this century as to what they wanted out of life, striving against the restrictions put upon them by governments and society. Maria Antonieta wanted to be President of the Republic when she was a child. However, this was one of her aspirations that didn’t seem to be very plausible at the time. Women has just won the right to vote in 1949, and hardly any had been accepted into government offices up until the later 1900’s. Most women were expected to have the same dream of getting married by age twenty and raising a large family. This role alone was difficult when the government of Chile during the 1970’s posed many hardships after the nationalizing of industries, banks, and mines. Many women had to hoard their goods such as alcohol, cotton, or wool trying to keep the necessities needed for taking care of their families. This angered many women and created many protests between the right and left wing governments. In 1973 Salvador Allende was killed during the coup and General Pinochet took power with help from the U.S. The military captured many remaining leftist supporters, forc ing people to flee or disappear. As in many countries with the military taking over, some people were detained or tortured, and some never seen again while leaving their families behind. Doctor Maria Matamala lived through some of this torture. She and a friend returned to the place where men gauged and electrocuted them, remembering all the suffering they had gone through as women of a machismo society. Around the 1980’s, many women came out of silence and demanded truth and justice for... Free Essays on In Womens Hand Free Essays on In Womens Hand Summary of â€Å"In Women’s Hands† During the 20th century, not only were many countries of Latin America undergoing stages of political instability with many governments having oppressive military rule, but women specifically were demanding more rights and respect inside and outside of the home. In Chile, women were becoming more assertive throughout this century as to what they wanted out of life, striving against the restrictions put upon them by governments and society. Maria Antonieta wanted to be President of the Republic when she was a child. However, this was one of her aspirations that didn’t seem to be very plausible at the time. Women has just won the right to vote in 1949, and hardly any had been accepted into government offices up until the later 1900’s. Most women were expected to have the same dream of getting married by age twenty and raising a large family. This role alone was difficult when the government of Chile during the 1970’s posed many hardships after the nationalizing of industries, banks, and mines. Many women had to hoard their goods such as alcohol, cotton, or wool trying to keep the necessities needed for taking care of their families. This angered many women and created many protests between the right and left wing governments. In 1973 Salvador Allende was killed during the coup and General Pinochet took power with help from the U.S. The military captured many remaining leftist supporters, forc ing people to flee or disappear. As in many countries with the military taking over, some people were detained or tortured, and some never seen again while leaving their families behind. Doctor Maria Matamala lived through some of this torture. She and a friend returned to the place where men gauged and electrocuted them, remembering all the suffering they had gone through as women of a machismo society. Around the 1980’s, many women came out of silence and demanded truth and justice for...

Seed priming technique Essays

Seed priming technique Essays Seed priming technique Paper Seed priming technique Paper Abstraction Seed priming is a technique by which seeds are partly hydrated to a point where sprouting processes begin but extremist outgrowth does non happen. Priming can be found effectual both for leguminous plants and grain harvests. A pot experiment was conducted under green house conditions at Pir Mehr Ali Shah, Arid Agriculture University, Rawalpindi during summer of 2007. The seeds were invigorated by traditional soakage ( hydropriming ) , osmo-conditioning ( soaking of seeds in aerated, low-water-potential solutions ) utilizing, potassium di-hydrogen phosphate KH2PO4, Mannitol, Polyethylene ethanediol ( PEG ) , sodium molybdate ( Na2MO4.2H2O ) and hormonal priming by utilizing salicylic acid ( SA ) . The scopes of osmotic potency for all the priming interventions were -0.5 to -1.2 M Pa. All the animation interventions significantly affected works energy, biomass, root, shoot length and nodulation. Osmopriming utilizing P @ 0.60 % applied in the signifier of KH2PO4 significantly improved seed energy in footings of average outgrowth clip ( 5.52 to 4.51 yearss ) , concluding sprouting per centum ( 74 to 89 % ) root and shoot length and nodulation ( 0 to 4 nodules seedling-1 ) followed by Osmitrol priming ( Mannitol @ 2 % ) . Overall all the seed priming techniques significantly improved the energy of mungbean seedlings as comparison to command. The usage of polythene ethanediol is expensive and gave similar consequences as for dry seeding so it should be replaced by hydropriming in farther experiments. It is recommended that nutrient-priming and osmopriming can be used as effectual tool for animation of mungbean seeds, for better growing and seedling constitution. Cardinal words: Osmo-priming, hydropriming, Vigna radiata, seedling energy, nodulation Introduction Seed priming is a pattern by which seeds are partly hydrated to a point where sprouting processes begin but extremist outgrowth does non happen ( Harris et al. , 2000 ) . Seed priming can be found effectual for legumes i.e. , outputs of Mungbean and Chickpea were increased well by priming seeds for 8 H before seeding ( Harris et al. , 1999 ; Musa et al. , 2001 ; Rashid et al. , 2004 ) . Mungbean ( Vigna radiata ( L. ) Wilczek ) is grown on over 200,000 hour angle with production of more than 100,000 T under rainfed and irrigated conditions in Pakistan. Outputs for the rainfed country are by and large low and variable due to sparse, fickle rainfall and fringy dirts. Mungbean production in Punjab Province is dependent chiefly on surface irrigation but it is besides grown under rain fed conditions. In the Southern part of Pakistan rainfall is bare and mungbean is grown with surface irrigation merely. Poor harvest constitution is a major restraint for mungbean production ( Naseem et. al. , 1997 ; Rahmianna et al. , 2000 ) and high outputs can be associated with early energy ( Kumar et al. , 1989 ) . Improved seed animation techniques are being used to cut down the sprouting clip, to acquire synchronised sprouting, better sprouting rate, and better seedling base in many horticultural ( Bradford et al. 1990 ; Rudrapal and Nakamura 1998 ) and field harvests like wheat, corn ( Aquilla and Tritto 1991 ; Basra et Al. 2002 ) and more late rice ( Farooq et al. 2004 ) . These animation techniques include hydropriming, osmoconditioning ( Basra et al. 2005 ) , osmohardening ( Farooq et al. 2006 ) and indurating ( Farooq et al. 2004 ) . These interventions can besides be employed for earlier and better babys room base constitution ( Lee et al. 1998 ) . This survey was initiated to research the effects of aerated hydration, hormonal priming ( salicylic acid ) , alimentary priming ( Phosphorous and molybdenum burden ) and osmo-conditioning on mungbean ( vigna radiata ) seed energy under green house conditions. MATERIALS AND METHODS Seed stuff Seeds of mungbean cultivar Chakwal Mung-97 ( CH-MUNG 97 ) were obtained from Barani Agricultural Research Institute ( BARI ) , Chakwal. The seeds were sterilized by utilizing 30 % hypochlorite for five proceedingss and so washed three times with distilled H2O. Seed Treatments The following seed priming interventions were applied Food priming The seeds were soaked in aerated solution of phosphoric ( P @ 0.60 A ; 1.20 % ) and molybdate ( Mo @ 0.02 A ; 0.04 % ) . The beginnings for phosphoric and Mo were potassium dihydrogen phosphate ( KH2PO4 ) and sodium molybdate ( Na2MoO4.2H2O ) , severally. Osmopriming The seeds were soaked in aerated solutions of Osmitrol ( mannitol @ 2 % A ; 4 % ) and polyethylene glycole ( Polyethylene glycol @ 5 % A ; 10 % ) . Hormonal priming The seeds were soaked in aerated solution of salicylic acid ( SA @ 10 A ; 20 ppm ) Post intervention operations After seed treatments the seeds were given surface rinsing three times by distilled H2O. Aeration was besides applied by pump. Air dried dirt was placed in 10-cm tall plastic pots with 6-cm diameter. The dirt used in the pot experiment was flaxen loam holding pH of 7.9. Dirt was made crumbly by rubing the surface with a two centimeter broad table fork to a deepness of 1-cm of moist dirt. The seeds were besides inoculated before seeding. Ten Numberss of seeds were planted in each pot and thinned to six workss per pot. Similar sum of H2O applied in regular intervals to all pots under survey. The workss were harvested 21 yearss after seeding ( DAS ) , and seedlings root/shoot length were taken. The seedlings were dried at 75 oC for 48 hours and the dry affair was eventually determined. The complete randomized design ( CRD ) was used in pot experiment. Analysis of discrepancy ( ANOVA ) was used to compare intervention agencies. Consequence The information showed that different seed priming interventions had important ( p lt ; 0.05 ) consequence on average outgrowth clip ( MET ) . The average outgrowth clip decreased with the application of seed priming interventions. Maximal average outgrowth clip ( 5.52 yearss ) observed in T1 ( control ) where dry untreated seeds were sown. Minimum ( 4.51 yearss ) MET was observed in T5 ( P @ 0.06 % applied in the signifier of KH2PO4 ) . All the interventions resulted in lower MET as comparison to command ( 5.52 yearss ) . The information besides revealed similar tendency with regard to fifty per centum ( E50 ) seeds to emerge as of MET. The lowest E50 was observed in T5 ( P @ 0.60 % ) . There was important ( p lt ; 0.05 ) consequence of different seed priming techniques on dry weight of 21 yearss old seedlings ( Fig 1 ) . The maximal fresh and dry weight was obtained in T5. All the priming interventions increased the fresh and dry weight of the seedlings except T2 and T12. Equally far as concluding sprouting is concerned maximal sprouting was achieved in T5 ( P @ 0.60 % ) . The lowest sprouting per centum was observed in T11 and T12 where polythene ethanediol was applied at the rate of five ( 5 ) and ten ( 10 ) per centum ( Table 1 ) . There was important ( p lt ; 0.05 ) consequence of different seed priming techniques on nodule formation and figure of 21 yearss old seedlings ( Fig 1 ) . All the priming techniques significantly increased the nodulation of seedlings as comparison to command except T10 and T12. Maximum nodule figure was observed in T5 ( P @ 0.60 % ) followed by T3 ( Mo @ 0.02 % ) . The information besides depicts that T9 and T11 are at par and T1, T10 and T12 gave similar consequences. The information depicts that seed priming had important impact on the root every bit good as shoot length, 21 yearss after seeding ( DAS ) . All the priming interventions significantly increased the root every bit good as shoot length of seedlings. The information revealed that T5 ( P @ 0.60 % ) and T9 ( mannitol @ 2 % ) gave the best consequences. The lowest root length ( 4.56 centimeter ) was observed in control. T4 ( Mo @ 0.04 % ) showed lower root length than T2 and T3 ( Mo @ 0.02 % ) and higher from control. Treatment T6 ( P @ 1.2 % ) showed lower root length than T5 ( P @ 0.06 % ) and at par with T1 and T4. Hormonal priming utilizing salicylic acid ( SA @ 20 ppm ) besides showed lower root length than T7 ( SA @ 10 ppm ) and at par with T4 and T6. The information besides showed that T10 ( mannitol @ 4 % ) is lower than T9 ( mannitol @ 2 % ) and at par with T7. The information besides depicts that T12 ( PEG @ 10 % ) showed lower root length as comparison to T11 ( PEG @ 5 % ) whic h showed besides higher root length than all other interventions except T2 and T9 ( Figure 1 ) . Maximal shoot length observed in the T5 ( P @ 0.06 % ) followed by T9 ( mannitol @ 2 % ) . The information besides depicts the shortest shoot length was observed in the control. The information besides revealed that T4 resulted in lower shoot length than T3 and T2. It is besides apparent that T6 is lower than T5 and at par with T3 and T2 ( Figure 1 ) . T8 showed higher shoot length than T7 and at par with T2, T3 and T6. Treatments T9 and T10 and T11, T12 besides showed similar consequences. Discussion Earlier and more unvarying sprouting and outgrowth was observed in fit seeds as indicated by lower MET and E50, higher sprouting per centum and root and shoot dry weight ( tables 1 ) . Lesser MET and E50 specify the earlier and rapid sprouting. These findings support the anterior work on canola ( Brassica compestris ) ( Zheng et al. , 1994 ) , wheat ( Triticum aestivum ) ( Nayyar et al. , 1995 ) and rice ( Oryza sativa ) ( Lee and Kim 2000 ; Basra et al. , 2003 ) who described improved sprouting rate and per centum in seeds subjected to hydropriming and seed hardening for 24 H. This survey revealed that osmo, food and hormonal priming could inspire mungbean seeds. One of the grounds for reduced MET is that during pre-sowing seed treatments the quiescence of the seed is broken and the seed bio-chemical procedures commences, which lead to faster sprouting and outgrowth ( Farooq et al. , 2006 ) . Seed priming ensured the proper hydration, which resulted in enhanced activity of a-amylase that hydrolyzed the macro amylum molecules in to smaller and simple sugars. The handiness of instant nutrient to the shooting seed gave a vigorous start as indicated by lower E50 and MET in treated seeds ( Farooq et al.,2006 ) during priming de novo synthesis of a-amylase is besides documented ( Lee and Kim, 2000 ) . Early outgrowth as indicated by lower E50 and MET in treated seeds may be due to the faster production of sprouting metabolites ( Saha et al. 1990 ; Lee A ; Kim 2000 ; Basra et Al. 2005 ) and better familial fix, i.e. earlier and faster synthesis of DNA, RNA and p roteins ( Bray et al. 1989 ) . Gray and Steckel ( 1983 ) besides concluded that priming increased embryo length, which resulted in early induction of sprouting in carrot seeds. The increased shoot and root length in fit workss can be due to metabolic fix of harm during intervention and that alteration in sprouting events i.e. , alterations in enzyme concentration and formation and decrease of slowdown clip between imbibition and radicle outgrowth ( Bradford et al. , 1990 ) . Treated seeds had stronger embryos that were able to more easy emerge from seeds ( Harris et al. , 2005 ) . These consequences are besides in line with the findings of Sekiya et Al. ( 2009 ) who reported enhanced root and shoot length of seedlings obtained from P enriched seeds. To lend to works growing and development seed priming has been widely reported technique ( Harris et al. , 2005 ) . Ajouri et Al. ( 2004 ) reported a stimulation of P and Zn uptake, every bit good as an improved sprouting and seedling growing in barley after soaking seeds in H2O and in solutions incorporating 5-500 millimeter P. It has been besides reported fresh seeds had higher energy degrees ( Ruan et al. 2002 ) , which resulted in earlier start of outgrowth as high energies seed tonss performed better than low vigour 1s ( Hampton and Tekrony 1995 ) . Yamauchi and Winn ( 1996 ) besides reported positive correlativity between seed energies and field public presentation in rice. Earlier, Zheng et Al. ( 2002 ) reported earlier and unvarying outgrowth in rice seeds osmoprimed with KCl and CaCl2 and assorted salts under afloat conditions. Hydropriming improved the early and vigorous harvest constitution in corn ( Nagar et al. 1998 ) and Helichrysum bracteatum L. ( Grzesik A ; Nowak 1998 ) . However, other surveies resulted in hapless outgrowth from hydroprimed Kentucky blue grass seeds under field conditions ( Pill A ; Necker 2001 ) . However Nascimento and West ( 1999 ) reported early sprouting of fit seeds but non recorded any betterment in the growing of seedlings in sweet melon seeds under research lab conditions. Confusing consequences, where priming did non demo any good consequences, besides reported by different research workers ( Mwale et al. , 2003 ; Giri and Schillinger, 2003 ) . The addition of nodulation, seedling energy and tolerance to emphasiss may depend on assorted factors happening during priming intervention. One hypothesis is that benefits of priming can be due to metabolic fix of harm during intervention and that alteration in sprouting events i.e. , alterations in enzyme concentration and formation and reduces lag clip between imbibition and radicle outgrowth ( Bradford et al. , 1990 ) . One of the possible grounds for early nodule formation may be due to enhanced activity of a-amylase and sucrose synthase in primed seeds which may ease works growing and energy ( Lee and Kim, 2000 ) . From the present survey it may be concluded that seed priming may heighten the seedling energy of mungbean. Nutrient priming utilizing phosphoric and osmopriming with Osmitrols were the most efficient priming interventions in this survey. In farther research work biochemical footing for the enhanced phenology of mungbean may be evaluated. Mentions Aquilla D. A. , V. Tritto. 1991. Germination and biochemical activities in wheat seeds following delayed harvest home, ageing and osmotic priming. Seed Sci.Tech.19:73 82. Anonymous.2009. Agriculture statistics of Pakistan. Ministry of Food, Agriculture and Livestock. Economic wing, Islamabad. Pp. 46-47. Association of Official Seed Analysis ( AOSA ) . 1998. Rules for proving seeds. J. Seed Tec.12:1-112. Association of Official Seed Analysis ( AOSA ) .1983.Seed Vigor Testing Handbook. Contribution No. 32 to the Handbook on Seed Testing. Barber, S. A. 1995. Soil alimentary bioavailability, 2nd edn. Willey, New York Basra, S. M. A. , M. Farooq, K. Hafeez and N. Ahmad. 2004. Osmohardening: A new technique for rice seed animation. Int. Rice Res.Notes.29:80 81. Basra SMA, Farooq M, Tabassum R. 2005. Physiological and biochemical facets of seed energy sweetening interventions in all right rice ( Oryza sativa L. ) . Seed Sci.Tech.33:623 628. Basra S. M. A. , M. N. Zia, T. Mehmood, I. Afzal, A. Khaliq.2002. Comparison of different animation techniques in wheat ( Triticum aestivum L. ) seeds. Pak. J Arid Agric.5:11 16. Basra, S. M A. , Farooq, M. and Tabassum, R. 2005. Physiological and biochemical facets of seed energy sweetening interventions in all right rice ( Oryza sativa L. ) . Seed Sci.Tech.33:623-628. Basra, S.M.A. , Farooq, M. and Khaliq, A.2003.Comparative survey of pre-sowing seed sweetening interventions in all right rice ( Oryza sativa L. ) . Pak. J. Life Soc. Sci.1:5-9. Basra, S.M.A. , Farooq, M. , Hafeez, K and Ahmed, N.2004.Osmohardening: A new technique for rice seed animation. Inter. Rice Res.Notes.27:74-75. Basra, S.M.A. , Farooq, M. , Tabassum, R. and Ahmed, N. 2005. Physiological and biochemical facets of seed energy sweetening interventions in all right rice ( Oryza sativa L. ) . Seed Sci.Tech.33: 623-628. Basra, S.M.A. , M. Farooq and A. Khaliq. 2003.Comparative survey of pre-sowing seed sweetening interventions in indica rice ( Oryza sativa L. ) . Pakistan J. Life Soc.Sci.1: 5-9. Basra, S.M.A. , Zia, M.N. , Mehmood, T. , Afzal, I. and Khaliq, A.2002.Comparision of different animation techniques in wheat ( Triticum aestivum L. ) seeds. Pak. J. Arid Agri.5:11-16. Bose, B. and Mishra, T. 1992. Response of wheat seed to pre-sowing seed interventions with Mg ( NO3 ) . Annals.Agri.Res.13:132-136. Bradford KJ, Steiner JJ, Trawatha SE.1990. Seed priming influence on sprouting and outgrowth of pepper seed tonss. Crop Sci.30:718 721. Bradford, K.J. , 1990. A H2O dealingss analysis of the seed sprouting rates. Plant Physiol.94:840-849. Bray C.M. , P.A. Davison, M. Ashraf, R.M. Taylor, Biochemical alterations during osmopriming of leek seeds, Ann.Bot.Lond.63:185-193. Bray, C.M. , Davison, P.A. , Ashraf, M. , Taylor, R.M. 1989. Biochemical alterations during osmopriming of leek seeds. Annalss Bot.36:185-193. Chojnowski M. , F. Corbineau, D. 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Sci. 9 ( 5 ) : 923-928 Ellis, R.A. and E.H. Roberts. 1981. The quantification of ageing and endurance in Orthodox seeds. Seed Sci. and Technol. 9: 373-409 Farooq M, Basra SMA, Cheema MA, Afzal I. 2006. Integration of pre-sowing soakage, chilling and heatingtreatments for energy sweetening in rice ( Oryza sativa L. ) . Seed Sci.Tech.34:499 506. Farooq M, Basra SMA, Hafeez K, Ahmad N. 2005. Thermal hardening: A new seed energy enhancement tool in rice. J.Integ.PlantBio.47:87 193. Farooq M, Basra SMA, Hafeez K, Warriach EA. 2004. The influence of high and low temperature interventions on the seed sprouting and seedling energy of coarse and all right rice. Int.RiceRes.Notes.29:69 71. Farooq M, Basra SMA, Hafeez K. 2006. Seed animation by osmohardening in all right and harsh rice. Seed Sci.Tech.34:181 187. Farooq M, Basra SMA, Karim HA, Afzal I. 2004. Optimization of seed indurating techniques for rice seed animation. Emir.J.Agric.Sci.16:48 57. Farooq, M. , Basra, S.M.A. , Hafeez, K and Ahmad, N. 2005.Thermal hardening: a new seed energy enhancement tool in rice. Ac.Bot.Sin.47:187-193. Farooq, M. , Basra, S.M.A. , Hafeez, K and Warriach, E.A. 2004.The influence of high and low temperature interventions on the seed sprouting and seedling energy of coarse and all right rice. Inter. Rice Res.29:69-71. Farooq, M. , Basra, S.M.A. , Tabassum, R. and Ahmed, N. 2006. Evaluation of seed vigour sweetening techniques on physiological and biochemical footing in harsh rice ( Oryza sativa L. ) Seed Sci.Tech.34:741-750. Farooq, M. , S.M. A. Basra and R.Tabassum.2006. Enhancing the public presentation of direct seeded all right rice by seed priming. Plant Prod. Sci.4: 446-456. Fu, J.R. , X.H. Lu, R.Z. Chen, B.Z. Zhang, Z.S. Liu, Z.S. Li, et al. , Osmoconditioning of peanut ( Arachis hypogen L. ) seeds with PEG to better energy and some biochemical activities. Seed Sci.Tech.16:197-212. Giri, G. S and W. F. Schillinger, 2003. 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Pros and Cons of Telecommuting

Pros and Cons of Telecommuting Pros and Cons of Telecommuting Pros and Cons of Telecommuting By Colin Telecommute (verb): to work from home, communicating with a central workplace using equipment such as telephones, fax machines, and modems. Telecommute; the word sounds as though it opposes the freedom that a freelance writing life should bring, and is therefore in no way desirable. But in an age when technology and e-commerce is being embraced by so many organisations, the word ‘telecommute’ has come to represent the key to a door of opportunity for freelance writers, more than any other word in the English dictionary before it. But like anything else in this world that sounds good, how much of that should be taken with a pinch of salt? Everything has a cost, so let’s take a look at the pros and cons, from a freelance writer’s perspective, of telecommuting. Pros Anywhere, Anytime Look at the word closely, and immediately you will begin to see what it has going for it. Tele – commute: the ability to â€Å"commute† anywhere in the world, without actually leaving the comfort of your own home. In other words, the world truly is your oyster. Being able to work for any company, anywhere in the world, opens up opportunities for freelance writers that were never dreamt of prior to the birth of the Internet. Effectively, it means that there are no longer any barriers to who can work for whom, and thanks to e-mail, communication between the employer and the employee is faster than it has ever been. Telecommuting cuts out the need for work permits, and gives employers a much wider scope to choose from, as they are no longer restricted geographically over whom they hire. Skill and talent alone, can once again be a major factor. Travel and the Environment Not owning a car – for whatever reason is no longer a barrier to being able to earn money. As a telecommuter, the only thing you need to get started is a PC, an Internet connection, a desk, a chair, and the motivation. If you are a believer in environmental causes, then telecommuting can allow you to earn while doing your bit for the environment. Global warming is a benefactor of telecommuting! Determine Your Own Schedule Freelance writing already allows you to determine your own working schedule. In the world of telecommuting, there is no such thing as 9 to 5. If you have a young family this can be of huge benefit, in that you can organise work around child care requirements, and shopping trips to purchase necessities. It’s also a huge benefit when dealing with companies on the other side of the world. For example, if a freelance writer in the U.K. gets contracted to a company in California, the time difference is negated because he can keep in touch through email. Cons Alienation Telecommuting means working from home can be as flexible as the freelance writer desires, but it also means the more jobs he gets from overseas, the more he can feel alienated on a much grander scale. We all know that writing is a solitary business, but when you are telecommuting on a global scale, the feeling that your home office is a small place to work is magnified to a much greater extent. It is therefore vital for all telecommuters to make sure they get plenty of vacation time and contact with the outside world. Sanity is a treasured commodity. Communication Dealing with clients in a telecommuting situation, also puts the freelance writer at risk of severe frustration if they are required to work with other writers on a project, especially if they are also spread around the world. Having no face to face contact with your client or team can often make the freelance writer feel vulnerable, because without the personal aspect, one mistake could be enough to see you dropped at the click of a button. Being on the other side of the planet to your client also means that although email is generally fast and reliable, there is always the scope that emails may be misinterpreted. For example, while you could read your brief in the wrong way and submit the wrong article, it also means your client may take something you say in entirely the wrong way, and end up being offended. Neither scenario is good if a long-term working relationship is at stake. Exchange Rates A freelance writer telecommuting for an overseas company that pays in a currency weaker than their home country, can be prone to feeling that money is being lost. For example, a freelance writer in the U.K. who earns $200 USD for a commissioned article, will receive around  £100 at the current exchange rate. It is therefore important to ensure you feel you are being paid fairly for the work you are required to submit. Technology Telecommuting means there is a heavy reliance placed on technology for all manners of things. These can range from merely keeping in touch with a client, to making submissions, look for work, and accepting payment. Suffer a PC crash or lose your interconnection, and you may as well be sitting on a raft in the middle of the Pacific Ocean. It is therefore vital that every telecommuter serious about their business, takes all precautions necessary to keep their PC and peripherals fully protected. The most vital things to remember are to have sufficient anti-virus protection software, good malware and adware scanning and removal software, a suitable firewall installation, and to take regular backups of all your data and software. It might also be a good measure to ensure your PC is regularly maintained, especially the hard drive, and that you never forget to pay your ISP bills. The decision to work in a telecommuting capacity can open doors to the most varied and exciting work a freelance writer can possibly imagine. Try it out; if you don’t like it, it costs nothing to stop – possibly the final pro of telecommuting. Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Freelance Writing category, check our popular posts, or choose a related post below:20 Computer Terms You Should KnowThat vs. Which50 Tips on How to Write Good

The Issue of Child Abuse in America Essay Example | Topics and Well Written Essays - 1000 words

The Issue of Child Abuse in America - Essay Example This situation depicts the kind of family dynamics that children today are experiencing in America. The American government should reform its child protection programs to stop child suffering within the family structure. Most American Parents have neglected their parental responsibilities as they pursue their personal goals without minding child welfare. In the Glass Castle, Walls (13) states that Brian had suffered from injury but his parents decided not to take him to the hospital just because there was another kid in the hospital. They said that â€Å"one child at a time in the hospital was enough†¦Ã¢â‚¬ , which depicts their ignorance and unwillingness to take care of the medical needs of their children. From such a careless statement, it is possible to point out that the parents neglected their parental responsibilities to ensure that children are healthy which is in accordance with children right of good health. Also, Brian’s mother, Wall, finds it worthy to engag e in painting rather than cook for the children. Consequently, children have to cook for themselves, without having to worry about the risk of fire that is associated with this task. In America today, most parents have placed more weight on the job and often leave children with the responsibility of fending for themselves. This abuse of children's rights is a manifestation of negative family dynamics that can only be corrected through a proper corrective reform in the children’s rights acts. In modern America, Children have to undergo tough conditions while conforming to the harsh environments that most parents subjected them to. Walls (17-20) notes the kind of life that her family lived due to the conditions of the environment that they lived. Rex, the family head, lives in a desert and keeps moving to ensure that he escapes his debtors. He moves the children in the middle of the night when he cannot be found by his collectors and the children hardly have a peaceful night. T hey have to sleep in the middle of the desert and acclimatize to the cold and the heat fluctuations of the environment. Shifting is a routine they have to keep up with to avoid any kind of misfortune that may befall their greatly indebted father. This makes it tough to live a comfortable life. In America, parents are always moving to new environments in search for better job subjecting their children to harsh living conditions. For instance, when families move, the children have to shift their from their schools every time making their academic life unstable and often unfriendly. In this regard, there is a need to protect children academic life by instituting stricter measures on parental care to safeguard children welfare. Parents who abuse drugs subject their children to physical as well as mental torture that often has a permanent effect on the children. In the glass Castle, Rex drinks a lot and does not mind the welfare of his children at all. When he takes the children to the f orest, he uses a commanding tone that scares them and subjects them to mental torture. When he doesn’t win in his gambling game, the children have to go hungry while he spares nothing for them to sustain his drinking habit.  

Language and Communication for Science Essay Example | Topics and Well Written Essays - 1250 words

Language and Communication for Science - Essay Example The Therefore, following the relationship between language and scientific development, I intend to expound on this relation. More to this, I shall give an examination of what this relationship implies in human resources development planning, and use Japan and China as illustrations. Scientific knowledge refers to objective knowledge of the universe and its phenomena. Such knowledge derives from the use of scientific methods of inquiry, and goes through public validation process to ensure it conforms to the empirical observations of natural phenomena. Storing such knowledge for further scientific advancement, as well as reference leads to the generation of scientific literature, which can be defined as a store of objective knowledge. Consequent to this, a clear relation develops between science and language as the knowledge developed gets to be stored in a certain form of language. Having elaborated on this relationship, I continue by highlighting the importance of language in the process of modernization. Scientific literature provides scientists with an accumulation of scientific knowledge. Given that scientists do not function in isolation, such literature provides them with the necessary information to boost their research. They refer to the scientific literature, to gain an understanding of what research has been done in the past. In addition to this, they get to develop knowledge gaps in such scientific knowledge, thus advancing the research process. Sharing the information with other scientists, using language as a means of communication, helps in innovating technologies that lead to the modernization of underdeveloped and developing nations. Given that scientists derive the required scientific information from scientific literature, the time taken to access the information determines the viability of any research

Fred Jones Constructon Essay Example | Topics and Well Written Essays - 2500 words

Fred Jones Constructon - Essay Example Insofar as the products are concerned, there is also a need to define the branding strategy thast can compete against other brands in the market. And in order to be competitive, there should be some awareness of the competitors’ strategies against which the Fred Jones Construction strategies for storm roof repairs will strive to be more captivating to potential customers. I.Industry & Market Analysis The storm roofing business realized a boom in Dallas right after a tornado touched down in April 2-3.2012 and a year ago in April 27, 2011. $ 100 million worth of insurance claims had been reported in 2011 for Dallas alone, based on the news report of Nielsen and Richter (2012). There were 19 separate warnings about a tornado approaching Dallas population with 6.3 million people. Hail brought by the powerful wind could be as small as peas and as big as a baseball. In mid-June 2012, Dallas experienced â€Å"the massive hail storm† which was estimated to have resulted in $ 2 billion worth of insurance claims (Richter and Berkowitz 2012). And sop, even if 1,000 contractors of roof repairs are tapped to fix all roofing and housing repairs, each contractor would have a potential market of about $2 million. One of the major industry players, MyRoofDfw (2013) in the roof repair industry within Dallas Forth Worth Metroplex disclosed that there are more than 1,000 companies offering the same products and services in the location. But that company also forecasted only about 10% will remain operating within the next 10 years. That company boasts of having the expertise at roof replacement, leak repairs, insurance claims assistance, lowest pricing, free estimates, and free consultancy for home roof condition reviews. Aside from these, the company offers more than a dozen related services listed in its website. Six of those services are free because those are about inspections pertaining to different reasons for repairs. Competitors like Circle H Roofing & Constru ction have communicated to the public the problem of unpredictable weather changes that have damaged roofs due to hail, fallen trees, and strong winds. They have set the customer’s expectations (Circle H 2013) way ahead of the customer’s decision to secure the products and services from their respective companies. This is good because the homeowners can also plan ahead whatever would have to be prepared by the time the products and service crews arrive. Companies offering both products and services are transparent about schedules, how customers may pay through financing, and what repairs are to be expected. They also offer free inspections and quotation. Their products and services are all with warranty from the company that fixed the problems. Furthermore, they assist homeowners in claiming for property damages due to storms. Another competitor, Bennet Roofing (2013) gives importance to the presence and availability of its â€Å"experienced, professional staff† and the capability to â€Å"install and repair all types of residential and commercial roofing† aside from offering free inspection of roof damages. One of its strategies for storm roof repairs is the combination of educating the customers using their website about what needs to be done in order to claim for damages from insurance companies and then to mention towards the latter part that Bennet Roofing offers to perform all those long, tedious step-by-step process for

Hydrosphere, Biosphere & Lithosphere Essay Example | Topics and Well Written Essays - 1250 words

Hydrosphere, Biosphere & Lithosphere - Essay Example Explain the relationship between the saturated zone, the water table, a ground water well and the cone of depression, all within the sub-surface. In the hydrological cycle, the water circulates between the oceans, the atmosphere and the continents. The water can exist in any of the three states during the transportation, i.e. as vapor expressed as humidity, as liquid in the form of droplets or as solid such as in the form of ice crystals or snowflakes. During the various process involved in the hydrological cycle, the quantity of water or 'water balance' that changes between inflow and outflow can be estimated based on the principle of conservation of mass. The movement of the water including the formation of water vapor is driven mainly by solar energy although other forces are also involved. The water evaporates from the surface of the ocean during the vapor phase. It then usually either falls as rain onto the surface of the earth (liquid phase) or as snowfall (solid phase) under t he process of precipitation, and on land a large portion of it usually flows in rivulets and rivers from the mountain tops to the seas in liquid form under the influence of gravity (surface runoff). Some of the water reaching land surfaces is also absorbed by the soil (infiltration) which can then follow one of several paths such as directly to the atmosphere by plants through transpiration. Within the sub-surface, a 'saturated zone' develops near the water table which is the surface that separates this zone from the zone of aeration (Monroe et al., 2006: 524). The saturated zone develops from the capillary rise of water in the pores of the soil, sediments and rocks. When a water well is used to pump the groundwater, the lowering of the water table in the area of the well can create a 'cone of depression', which refers to the loss of water having exceeded its inflow. 2.The food chain is a valuable concept in biogeography. Give an example of a specific food chain, labeling the variou s levels of the food chain. After looking at characteristics of food chains, explain how a geographer’s approach to the study of organisms might be different than biologist’s study of organisms; what would each try to emphasize more than the other? What exactly is a biome? Compare/contrast the concept of the biome with that of the zoogeographic region. Compare/contrast the floral characteristics of 2 of the following biomes: Desert, Tundra, Midlatitude Grassland and Boreal Forest. An example of a simple three-step specific food chain is given below with the deer as the herbivore and the lion as the carnivore. Food chains indicate a unidirectional transfer of energy beginning with the producers (as autotrophs) and ending with the topmost carnivores. Knowing the food chains help in identifying the interactions and interdependence between living organisms. The distributions of the energies and biomasses in each food chain are typically though not always pyramidal. In real ity, many food chains have at least four steps and several are intricately linked forming food webs. Grass (producer) > Deer (herbivore) > Lion (carnivore) In studying the characteristics of food chains, a geographer would be more interested in the wider ecosystems; in how the components of the chain are interdependent, in the flow of energy through the whole system between the different trophic levels, in examining the levels of biomass, and so on. A biome is a natural ecological grouping of animals and plants on the basis of climates, i.e. â€Å"all the ecosystems taken together in a given geographical area having the same type of climate† (Bharatdwaj, 2006: 100). Other environmental factors could also differentiate biomes but they are

Investment Strategy and Portfolio Management - 2 Essay

Investment Strategy and Portfolio Management - 2 - Essay Example The move towards conservatism is a reaction to the recent crisis, as more people worldwide felt that not enough regulation was exercised by the government to control risky instruments and markets. However, there is also a possibility that a more protectionist attitude may characterize the new administration, which may hamper economic growth. The more conservative economic policy may work either way; it could provide a more stable environment for business by eliminating risks, but at the same time it might limit business opportunities. Thus new directions must be carefully watched post-election. According to the Wall Street Journal report, this year’s treasury’s budget deficit forecast, at 12.6%, remains to be the highest in the nation’s history, while forecasts for 2011 to 2011 economic growth appear to optimistic. Tax receipts remain weak because of the poor earnings level in both the business and household sectors of the economy (Norman, 2010). However, the Office of National Statistics reported that the UK economy actually grew by 0.1% for the last quarter of 2009, the first following six quarters of economic contraction. It may not be conclusive that the recession is ended, but it does provide a hopeful sign which may be confirmed in the coming quarters (Angus Reid, 2010) According to Price (2010), the current high levels of US government debt is a cause for worry. The uncertainty in the possible outcome of the credit â€Å"tug-o-war† itself exerts pressure on the market to perform with greater volatility (erratic price movements). Price notes that the supply of gilts is moving upwards consistently, and together with it the UK’s credit fundamentals will continue to quickly deteriorate. What would be worse is the threatened debt downgrade from one of the ratings agencies. This could mean that the UK will be able to source additional debt only at higher interest

Was the US justified in using the Bomb against Japan Essay

Was the US justified in using the Bomb against Japan - Essay Example The bombing was necessary to prevent massive causalities on either side in the planned invasion since it precipitated the surrender. Japan’s plan was to inflict very heavy losses, which the war-weary Americans would not stand; neither would they negotiate for peace. Had the US continued with the plan to land on Kyushu, they would have suffered horrendous causalities. Pre-inventing causalities approximation anticipated the loss of between 100,000 to 1 million American soldiers, with 5 to 10 million Japanese civilians and military losing their lives ( Chappell 145). It has been estimated that between 250,000 to 400,000 Asians under Japanese occupation would have lost their lives for every month that the war went on. In this context, the fact remains that the atomic bombing against Nagasaki and Hiroshima resulted to the end of the World War II much sooner than any other alternative that could have been used and in so doing, millions of lives were saved. Given the reason that the Japanese had caused 17 million deaths, it is justifiable that the atomic bombing on Japan was to end the war. According to President Truman, an atomic bomb was to be used on Japan without a warning. In a radio broadcast following the atomic bombing on the two cities, President Truman announced that he realized the disastrous implication of the atomic bomb, which they had used against those who attacked Americans without caution, at Pearl Harbor. The bomb was to attack those who have beaten and starved and executed American war prisoners against those who deserted all the pretense of abiding by the international warfare law, and it was meant to shorten the misery of young Americans. The US was to continue using it until they totally destroy Japan’s power of making war. A decision of Japanese surrender was the only move that would have stopped them. The president was well aware of the savage way in which the Japanese waged war in

Investment Strategy and Portfolio Management - 2 Essay

Investment Strategy and Portfolio Management - 2 - Essay Example The move towards conservatism is a reaction to the recent crisis, as more people worldwide felt that not enough regulation was exercised by the government to control risky instruments and markets. However, there is also a possibility that a more protectionist attitude may characterize the new administration, which may hamper economic growth. The more conservative economic policy may work either way; it could provide a more stable environment for business by eliminating risks, but at the same time it might limit business opportunities. Thus new directions must be carefully watched post-election. According to the Wall Street Journal report, this year’s treasury’s budget deficit forecast, at 12.6%, remains to be the highest in the nation’s history, while forecasts for 2011 to 2011 economic growth appear to optimistic. Tax receipts remain weak because of the poor earnings level in both the business and household sectors of the economy (Norman, 2010). However, the Office of National Statistics reported that the UK economy actually grew by 0.1% for the last quarter of 2009, the first following six quarters of economic contraction. It may not be conclusive that the recession is ended, but it does provide a hopeful sign which may be confirmed in the coming quarters (Angus Reid, 2010) According to Price (2010), the current high levels of US government debt is a cause for worry. The uncertainty in the possible outcome of the credit â€Å"tug-o-war† itself exerts pressure on the market to perform with greater volatility (erratic price movements). Price notes that the supply of gilts is moving upwards consistently, and together with it the UK’s credit fundamentals will continue to quickly deteriorate. What would be worse is the threatened debt downgrade from one of the ratings agencies. This could mean that the UK will be able to source additional debt only at higher interest

Comparison of The Speckled Band and Lamb to the Slaughter Essay Example for Free

Comparison of The Speckled Band and Lamb to the Slaughter Essay In this essay I will be comparing the two stories The Speckled Band by Sir Arthur Conan Doyle and Lamb to the Slaughter by Roald Dahl. The Speckled Band was written in 1892.This was in The Victorian Period when people had very little faith in the Police and Conan Doyle needed to create a Detective who always got everything right so that the public started to believe the Police could actually get it right. It was first published in a magazine called The Strand in weekly episodes so to a certain extent he needed to keep his readers interested as well which is why the plot is so complicated. The plot is about a woman called Helen Stoner who is going to get married, but someone or something murders her sister who also was supposed to get married. Helen Stoner goes to see Sherlock Holmes to see if he can help solve the crime. The plot is full of twists and red herrings that is up to the reader to try and solve as they go along but as always Sherlock Holmes works out the significance of everything and solves the crime. Lamb to the Slaughter was written in 1954by Roald Dahl. This story was also published in a magazine called Harpers but was written to be read in one go so it does not have the complicated layout of Conan Doyles story. Instead the reader knows exactly what is going on form the start of the story and the ending is shocking and designed to entertain and horrify. It was written by Dahl to show how women at the time were a lot more powerful than the men gave them credit for and should be respected more than being at home , pregnant. The plot is about a modern couple that seem to be falling out. Mary Maloney is the perfect 1950s housewife who makes dinner and cleans, but one day when her husband (Patrick Maloney) comes home from work they have an argument. Mary loses her temper with Patrick and hits him over the head with a leg of lamb and accidentally kills him. In a panic she phones the police and puts the leg of lamb in the oven to cook for dinner. When the police arrive she persuades them to eat the lamb because it would be a waste of food. In the end the police eat the evidence and Mary didnt get caught. Dahl uses comic irony at the end to engage the reader as Mary sits there laughing about what she has done; you dont imagine her to do this as a middle class housewife. The times that these stories are set in are both very different. Like travel, in The Speckled Band. They travel by horse and cart but in Lamb to the Slaughter trey use car or foot just like in the modern day. For communication the people in 1892 would have to write or go and see the person they wanted to talk to but in 1954 they would have used the telephone. Also in 1892 they did not have much electricity so they used candles instead of light, whereas in 1954 they had lots of electricity. In 1892 the women would have worn veils and long skirts but in 1954 they would have they would have worn jeans, t-shirt etc. in 1892 rich families would lived in large houses with servants but in the modern day most families are middle classed and do not have the money for servants etc. When there was an enquiry in 1892 the detectives would have had to use their own knowledge to solve crimes but in 1954 they would use fingerprints, evidence etc to solve the crimes. The language used in The Speckled Band was old fashioned and very formal like when Sherlock Holmes says Very sorry to knock you up Watson, said he, but its the common lot this morning. He also says things like Mrs Hudson has been knocked up, she reported upon me, and I on you. This isnt the kind of language we would use in the modern day and proves that it is set in 1892. However in Lamb to the Slaughter there was a lot of slang and it was not as formal. Like when Mary says Hullo darling. And Hullo Sam to the grocer. However the way she speaks to Sam the grocer proves it was set in the 1950s and not in any other time because we would not know the person in the corner shops first name today but it was common in the 1950s to be friendly with them. In The Speckled Band Sherlock Holmes is the main character. He is a famous detective and solves difficult cases. Conan Doyle invented him because people in the Victorian times were fed with the fact that the police could not solve any crimes and he wanted a character that always got everything right. However in Lamb to the Slaughter there were police who are good at solving cases but wouldnt be able to solve them without any evidence and they are very arrogant and treat Mary as a silly woman when in fact she is the person who has tricked them and been the murderer. Dahl does this to shock his readers and make it even more dramatic which Conan Doyle would not want to do because they needed to trust their detective! In The Speckled Band the killer was a snake that had been hypnotised by Helen Stoners stepfather, but all the way through the story he is a suspect and he acts suspiciously like when Helen goes to visit Sherlock Holmes to tell him what has been going on, shortly after she leaves her stepfather came in asking if she has been here. It seemed like he had something to hide, but in the end it had nothing to do with him. In Lamb to the Slaughter Mary seems like a nice, gentle woman who feels that she has to help out. Like when she says, Darling, shall I get your slippers? but after the police eat the evidence she laughs so I think he whole issue hade made her go a bit insane. At the beginning of the story you didnt expect it from her because she just seems like a normal, average wife like when it said about She took his coat and hung it in the closet. and when it said She was sat back again in her chair with the sewing. The scene seems too realistic for something bad to happen. In The Speckled Band the victim was Helen Stoners sister because there was nothing anyone could do to save her; this made the readers feel very sorry for her. The next victim was Helen Stoner and because nobody knew what killed her sister it seemed that she needed protection. I would expect someone like her to be the next victim because she is a woman who has done nothing wrong and is innocent. I dont think her or her sister deserved to be the victims because they are both just happy and normal people. In Lamb to the Slaughter you dont feel sorry for Patrick because he is being horrible to Mary and you feel automatically on her side from the beginning. I think he deserved to be upset but not killed because they are just having a falling out. The Speckled Band is set in Stoke Moran, which is Helens stepfather house. Sherlock Holmes is investigating the house. I think it is a good setting for a murder to take place and because its a very big house which makes it creepier for the reader because you dont know what is doing the killings. Lamb to the Slaughter is set in Mary Maloneys house. I think the way the author has described it makes it sound very realistic, like when he says, The room was warm and clean, the curtains drawn, the two table lamps alight. This makes the room sound cosy and like it really exists. You can really picture the scenery. I also think its a good setting for a murder because you can imagine it happening there. I think I would expect it because so many crimes in real life have been taken place in houses. Out of the two stories I preferred Lamb to the Slaughter because it is easier to understand and keeps you interested all the way though with the description it uses. I disliked the other story because the speaking parts dragged on to long and I did really just want to get on and find out what happens.

Cell Culture and Protein Detection

Cell Culture and Protein Detection Overview: This practical is split into three main parts, each part allows us to develop certain techniques. The first part of the practical is focused on the techniques needed to successfully carry out cell cultures. The second part is using an analytical technique known as ELISA this is used to measure the amount of proteins secreted by the cells that were cultured in part one. Lastly, part three focuses on western blotting, this is a technique used to measure proteins too but it differs from ELISA in that it measures proteins that are present inside the cell. The fundamental objective of this practical is to look at how Bacterial lipopolysaccharide (LPS) alters the growth and the expression of the smooth muscle cell ÃŽ ±-actin (SMA) in vascular smooth muscle cells (vSMC) while also detecting and quantifying cell signalling molecules (JNK’s) and cytokine secretion (TNF-ÃŽ ±) in vascular cells. Section 1 – Subculture Objective: The aim of this part of the practical is to subculture the bovine aortic vascular smooth muscle cells and to count the cells and check for viability by counting the cells in suspension on a haemocytometer using a microscope. The cells are also treated with a bacterial antigen (LPS) to look at its effect on viability, differentiation state and the activation of intracellular signalling and secretion of proteins. Immunocytochemistry is also carried out in this part of the practical. Method: Trypsin is used to remove the cells from the bottom of the flask. When the cells have dissociated, medium which contains a trypsin inhibitor is added. The medium stops excessive trypsin action from damaging the cells. The cell suspension is first diluted using trypthan blue to allow viable cells which remain white to be distinguished from non- viable cells which are blue. This method allows us to get a total cell count of cells/mL and the percentage of viable cells. Our total cell count was found to be 7.45105 cells/mL The non-viable cells were found to be 1.5104 cells/mL This means that the culture contains more 98% viable cells which indicates that it is a healthy culture. 3 different 6 well plates were then set up using different densities of seed cells. 3105cells/ml seeding density was used in one plate. This density was used as a lot of protein needs to be present for the assessment of JNK and ÃŽ ±-actin protein cytokine production. 1105 cells/ml seeding density was used in another 6 well plate. These plates are used to assess the effect of LPS on growth and viability of the cells. This density is used to give a clear picture of the cells to make counting easier. 5103 cells/ml seeding density was used in the last plate. This plate is used for ÃŽ ±-actin expression by immunocytochemistry. The low density will give us a clearer picture. The three different sets of plates are all treated with varying concentrations of LPS. Each plate has two wells which are used as controls, containing no LPS, two wells containing 1Â µg of LPS and two wells containing 10Â µg of LPS. Immunocytochemistry is carried out on the plate with a seeding density of 5103cells/ml. This technique is used to determine if a particular protein or antigen is present. An unlabelled primary antibody is used to bind to the antigen desired antigen. The presence of contractile protein ÃŽ ±-actin can determine the differentiation state of vSMC. Immunocytochemistry is used as a qualitative method of determining the presence of a protein, it is not quantitative. Results: Calculations for different seeding densities: Initial concentration = 7.45105 cells/mL Formula = Plate 1, seeding density 1.5105 cells/ml Plate 2, seeding density 0.5105 cells/ml Plate 2, seeding density 2.5103 cells/ml Table 1: Cell growth for each group Immunochemistry results: Figure 1: Cell growth in the control (0Â µg/ml LPS) Figure 1: Cell growth in the control (1Â µg/ml LPS) Figure 3: Cell growth with 10Â µg/ml of LPS Discussion: The first part of this section was to carry out a cell count and determine the viability of the suspension. Our sample had 7.45105 cells/ml and it contained more than 98% viable cells. This meant it was a healthy cell suspension and it was suitable to run tests on for the practical. A seeding density of 1105 cells/ml seeding density was used to assess the effect of LPS on growth and viability of the cells. This density was used to give a clear picture of the cells to make counting easier. The results of our experiment correlated with the majority of the class. However there were some unexpected results in some of the groups but this could be down to plates being labelled incorrectly or mistakes while counting when using the haemocytometer. Immunocytochemistry is used to allow us to analyse the results visually. We found that as the amount of LPS increased the number of cells decreased, this is illustrated in figures 1, 2 and 3. LPS is an endotoxin and it inhibits the growth of ÃŽ ±-a ctin. LPS also damages the structure of ÃŽ ±-actin, high levels of LPS stop the correct formation of the filaments and so affects the function of the cell which is to facilitate cell contraction and migration. This result was expected as LPS is a major mediator to septic shock and is known to directly affect vascular smooth muscle cells. Question: What could you do to improve this experiment? To improve this experiment I would use a wider range of concentrations for LPS. This would give a better understanding of its affects. Repeating the experiment several times and getting an average of your results would also help. Section 2 – ELISA Objective: The aim of this section of the practical is to use Enzyme-Linked Immunosorbent Assay (ELISA) to detect production of the cytokine TNFÃŽ ± from the cells activated with LPS. Detection is based on a colour change. Firstly a standard curve must be generated so that absorbance values can be converted into concentrations of TNF-ÃŽ ±. Once the curve is completed we can determine the unknown concentrations of TNF-ÃŽ ± in our samples. Method: Firstly the antibody is immobilised onto the surface of the plate. The plate is then washed to remove any excess antibody, antigen is then added and it is allowed to bind to the antibody. A secondary antibody is then added, this antibody is labelled with an enzyme. The enzymes substrate is then added, this causes a colour change. The amount of coloured product formed is determined spectrophotometrically. The amount of coloured product is proportional to the amount of enzyme present and also to the concentration of the antigen. Results: Table. 2 Absorbance values of samples at 450nm. Figure 4. Plot of standard curve of absorbance versus concentration. Discussion: The aim of this experiment was to quantify the amount of TNF-ÃŽ ± present in our samples. To do this a set of known standards were used and their absorbance values read. This data produced a straight line with an R2 value of 0.99 indicating that a straight line was an excellent fit for absorbance versus concentration, and so the equation of the line could be used to determine unknown concentrations of TNF-ÃŽ ± based on their absorbance values. Our results showed that TNF-ÃŽ ± was present in its highest concentration of 25.15pg/ml in the sample with the highest concentration of LPS and it was found in its lowest concentration of 9.9 pg/ml in the sample containing no LPS. This result was expected as cytokines such as TNF-ÃŽ ± are produced in large quantities to respond to endotoxins such as LPS. Question: What could you do to gain more information from this experiment? To gain more information from this experiment you could test for other cytokines which are also activated by LPS, correlating these results would make your data more meaningful. Section 3 – Western Blotting Objective: The aim of this section of the practical is to prepare cell lysates from the vascular smooth muscle cells which were activated by LPS previously. SDS PAGE and western blotting will then be used to detect the activation of the intracellular protein JNK. Western analysis quantifies the amount of protein present in the cell. To do this cell lysis must be carried out. In this practical we used a method which generates whole cell lysates. To do this lysis buffer is added to the cells followed by sonication. Method: Cell lysis is carried out first. The next step is SDS polyacrylamide gel electrophoresis. Western blotting involves transferring the protein bands from an acrylamide gel to a more stable and immobilising medium such as nitrocellulose paper so that analytical procedures such as detection with antibodies can be carried out. We carried out western blotting using iBlot dry blotting system. After blotting probing is carried out to determine the presence of phosphorylated JNK protein. Results: Figure 5. Ponceau S Staining Figure 6. ÃŽ ±-actin Figure 7. pJNK Discussion: To see if our transfer was a success before probing, the blot was stained with Ponceau S stain. The proteins can be seen as red bands with this stain. The result of this stain can be seen in figure 5. red bands are present which indicates our transfer was a success and that there are proteins present. The western blot analysis showed that ÃŽ ±-actin was present in all the samples as a strong band around 42kDa was observed which is expected for ÃŽ ±-actin. The results for pJNK did not work out as it was washed incorrectly, because of this no bands were observed, however bands would be expected in the samples containing LPS. Why did you run the sample on the gel before blotting? The sample was run on gel first as proteins are separated by molecular weight. This allows us to distinguish ÃŽ ±-actin from other proteins. It is then moved to the nitrocellulose paper so that analytical procedures such as detection with antibodies can be carried out Why measure the phosphorylated form of JNK? The phosphorylated form of JNK is a signal a cell sends out when it is stressed. Therefore pJNK should be present in the samples with LPS. If it is present then it confirms the fact that the cell is stressed as a result of the presence of the endotoxin.